Isolating Mortierella alpina strains of high yield of arachidonic acid

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Arachidonic acid production by Mortierella alpina using raw crop materials.

BACKGROUND Arachidonic acid (ARA) is one of the three essential fatty acids, and it is important for human body to keep healthy and is widely used. At present, expensive materials such as glucose and yeast extract are generally reported to be optimal for ARA production. A new cost-effective fermentation process including cheaper material for ARA production is of great significance. METHODS Fe...

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Statistical Optimization of Arachidonic Acid Production by Mortierella alpina CBS 754.68 in Submerged Fermentation

Arachidonic acid is an essential fatty acid in human nutrition. In the present study, production of arachidonicacid by Mortierella alpina CBS 754.68 was evaluated in submerged fermentation. The fermentation variableswere selected in accordance with the Plackett-Burman (PB) design and further optimized via response surface methodology (RSM). Five significant variables, namely glucose...

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Statistical optimization of arachidonic acid production by Mortierella alpina CBS 754.68 in submerged fermentation

Arachidonic acid is an essential fatty acid in human nutrition. In the present study, production of arachidonic acid by Mortierella alpina CBS 754.68 was evaluated in submerged fermentation. The fermentation variables were selected in accordance with the PlackettBurman (PB) design and further optimized via response surface methodology (RSM). Five significant variables, namely glucose, yeast ext...

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Metabolic engineering of Mortierella alpina for arachidonic acid production with glycerol as carbon source

BACKGROUND Although some microorganisms can convert glycerol into valuable products such as polyunsaturated fatty acids, the yields are relative low due primarily to an inefficient assimilation of glycerol. Mortierella alpina is an oleaginous fungus which preferentially uses glucose over glycerol as the carbon source for fatty acid synthesis. RESULTS In the present study, we metabolically eng...

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Expression of Vitreoscilla hemoglobin enhances production of arachidonic acid and lipids in Mortierella alpina

BACKGROUND Arachidonic acid (ARA, C20:4, n-6), which belongs to the omega-6 series of polyunsaturated fatty acids and has a variety of biological activities, is commercially produced in Mortierella alpina. Dissolved oxygen or oxygen utilization efficiency is a critical factor for Mortierella alpina growth and arachidonic acid production in large-scale fermentation. Overexpression of the Vitreos...

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ژورنال

عنوان ژورنال: Letters in Applied Microbiology

سال: 2004

ISSN: 0266-8254,1365-2673

DOI: 10.1111/j.1472-765x.2004.01581.x